History
The first outline of the Equine Respiratory Tissue Biobank (ERTB) was drafted in 2006 in the Laboratoire de Biologie Cellulaire et Moléculaire Respiratoire of the Université de Montréal (Dr Lavoie) thanks to a successful collaboration with the Laboratoire de Télématique Biomédicale du RSR (LTB) (Dr Rousseau) and the QRHN Biobank (Dr Laviolette). The goal was to develop an efficient method of classification and archiving of physiological results the biological samples collected in well characterized diseased and healthy horses. This system has become essential for optimizing the sharing capabilities of the tissues samples between distant laboratories.
Origin of the tissues
The principle objective of the Equine Respiratory Tissue Biobank is to simplify the accessibility of tissues from well-characterized horses with respiratory diseases such as heaves and IAD. The Biobank archived also tissues of appropriate age-matched control horses.
The equine tissue samples are largely obtained from research projects having received accreditation by each institution animal care committee. The other samples are received from diagnostic procedure or euthanasia of a client-owned horses with permission from the owner.
Procedures and conservation
Uniformity in the collection and conservation of the samples is essential in obtaining valid results from studies having used materials from multiple research centers. The Equine Respiratory Tissue Biobank proposes standardized protocols to assure the uniformity of manipulations from the farm to the location of conservation, assuring quality for the specific needs of each research group. The Biobank ables the archiving of physiological measurements and other pertaining information to well characterize aliquot, extract, slide, pellet, or anatomic piece specimens as it functions in nature.
Extracts
The extracts are regrouped into 3 sub-categories, RNA, DNA, and proteins. The RNA extracts are categorized by the description of the extraction technique employed, the ratio 260/280 and the (RIN). The use of protease and phosphatase inhibitors is indicated clearly in the Biobank for protein related extracts. Furthermore, the stimulants used are indicated for all the extracts from cellular isolation.
Slides
The slides permit the conservation of cells from different sources of origin (blood samples, bronchoalveolar or tracheal lavages) for future use. The fixation (acetone/methanol, formaldehyde, sucrose and others) or coloring is chosen in function of the subsequent analyses foreseen. The Equine Respiratory Tissue Biobank ables to record this information and assure a uniformity in the nomenclature of the fixatives used.
Pellets and pieces
The pellet and the anatomic pieces also undergo different processes of fixation (acetone, formaldehyde, liquid nitrogen, and others) in function with their future use. They are conserved in different embedding material (OCT, paraffin, etc.). A macroscopic evaluation of the pieces is performed for the characterization of the samples. Again, the Biobank archives all this information which allows for the user to chose the samples which best corresponds to their needs.
Physiologic results
The web application which supports the Biobank allows to archive many physiological parameters related to the specimens. This information is regrouped into five sub-categories: Subject, housing, medication, prophylaxis and diagnostic tests.
Les sections « sujet » et « régie » contiennent les informations permettant de décrire l’animal, d’identifier sa provenance et de répertorier l’alimentation et la litière utilisée lors de la prise d’échantillons.
Les sections « médication » et « prophylaxie » permettent d’archiver les médicaments administrés à l’animal jusqu’à six mois avant la prise d’échantillons ainsi que l’historique de leur vaccination et vermifugation. La section « médication » regroupe les médicaments selon leur mécanisme d’action; corticostéroïde, anticholinergique, ß2-agoniste, anti-inflammatoire non stéroïdien, antibiotiques et autres. Ce mode de classification permet de faire des recherches par groupe médicamenteux et par nom générique de médicament.
La section « test » inclut les résultats de sept catégories de tests : l’examen clinique, la fonction respiratoire, l’endoscopie, les lavages, les tests sanguins, l’évaluation de la performance et les autres tests. La section sur la fonction respiratoire contient des informations particulièrement importantes pour bien caractériser l’animal lors du prélèvement. Elle permet d’archiver les informations obtenues à partir de différentes méthodes de caractérisation de la fonction pulmonaire; la fonction respiratoire de base, postprovocation, postbronchodilatation. La caractérisation macroscopique des voies respiratoires supérieures et inférieures est archivée dans la section endoscopie. Les résultats d’analyses de lavages bronchoalvéolaire ou trachéal se retrouvent dans la section des lavages. La section des autres tests permet de conserver des informations sur les radiographies thoraciques.
All those informations allow to characterize the subject at the time of sampling, which facilitates the future use of samples.
